Composite

Part:BBa_K5286008:Design

Designed by: Mara Valverde Rascón   Group: iGEM24_TUM-Straubing   (2024-10-02)


CAHS106094+pT7max


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1269
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7max works the best for this sequence


Source

This part was assembled by the 2024 TUM Straubing iGEM team

References

Boothby, T. C., Tapia, H., Brozena, A. H., Piszkiewicz, S., Smith, A. E., Giovannini, I., Rebecchi, L., Pielak, G. J., Koshland, D., & Goldstein, B., “Rapid and Scalable Preparation of Bacterial Lysates for Cell-Free Gene Expression“, ACS Synth Biol. 2017 Dec 15;6(12):2198-2208